Unmasking of bacteriophage Mu lipopolysaccharide receptors in Salmonella enteritidis confers sensitivity to Mu and permits Mu mutagenesis
نویسندگان
چکیده
منابع مشابه
Cloning of the A gene of bacteriophage Mu and purification of its product, the Mu transposase.
The bacteriophage Mu transposase (the Mu A gene product), which is absolutely required for both integration of Mu and replicative transposition during the lytic cycle, has been overproduced by cloning the gene on a plasmid under the control of the phage lambda PL promoter. The protein has been purified to near homogeneity from the lysate of heat-induced cells of a strain carrying the plasmid. T...
متن کاملReplacement of the bacteriophage Mu strong gyrase site and effect on Mu DNA replication.
The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108...
متن کاملTemperature-sensitive mutants of bacteriophage mu.
Temperature-sensitive mutants of bacteriophage Mu, which grow at 32 C but not at 42 C, have been isolated. These mutants fall into two groups. Group 1 mutants fail to lyse host cells at nonpermissive temperatures, whereas lysis occurs normally with the group 2 mutants. All of the group 1 mutants apparently belong to the cistrons mapping to the left of gene C, whereas the group 2 mutants have le...
متن کاملIntroduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.
The host range of coliphage Mu was greatly expanded to various genera of gram-negative bacteria by using the hybrid plasmic RP4::Mu cts, which is temperature sensitive and which confers resistance to ampicillin, kanamycin, and tetracycline. These drug resistance genes were transferred from Escherichia coli to members of the general Klebsiella, Enterobacter, Citrobacter, Salmonella, Proteus, Erw...
متن کاملSuperdeduction in Lambda-Bar-Mu-Mu-Tilde
Superdeduction is a method specially designed to ease the use of first-order theories in predicate logic. The theory is used to enrich the deduction system with new deduction rules in a systematic, correct and complete way. A proof-term language and a cut-elimination reduction already exist for superdeduction, both based on Christian Urban’s work on classical sequent calculus. However the compu...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1988
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.170.3.1076-1081.1988